VIABILITY OF CHAMOMILLA-RECUTITA (L) RAUSCHERT CELLS AFTER CRYOPRESERVATION

被引：0
作者：
CELLAROVA, E
CERNICKA, T
VRANOVA, E
BRUTOVSKA, R
LAPAR, M
机构：
来源：
CRYO-LETTERS | 1992年 / 13卷 / 01期
关键词：
CHAMOMILLA-RECUTITA; PRE-CULTURE; CRYOPRESERVATION; POSTTHAW TREATMENT; VIABILITY;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Habituated callus cultures of Chamomilla recutita (L.) Rauschert, cell line K2-27-7 were pre-cultured on medium supplemented with ABA or GA3 for three subcultures and cryopreserved in the presence of a cryoprotectant mixture containing DMSO, glycerol and sucrose. Survival of the cells was determined at three time intervals by TTC test. It was found that both precultured and control samples require approximately 10 weeks for successful regrowth. After this period, most of the samples did not show any significant differences in cell viability. Beneficial effect of post-thaw treatment that consisted of washing with fresh medium and addition of activated charcoal to the culture medium on the viability of cells has not been confirmed.
引用
收藏
页码：37 / 42
页数：6
相关论文
共 50 条
[41]   Evaluation of the viability of Pleurotus spp. strains after liquid nitrogen cryopreservation [J].
Lara-Herrera, I ;
Mata, G ;
Gaitán-Hernández, R .
REVISTA DE MICROBIOLOGIA, 1998, 29 (03) :193-196
[42]   Viability and drug metabolism capacity of alginate-entrapped hepatocytes after cryopreservation [J].
L. Rialland ;
C. Guyomard ;
M. Scotte ;
C. Chesné ;
A. Guillouzo .
Cell Biology and Toxicology, 2000, 16 :105-116
[43]   FACTORIAL DESIGN TO OPTIMIZE THE VIABILITY OF THE ALGA SCENEDESMUS-SUBSPICATUS AFTER CRYOPRESERVATION [J].
BENHRA, A ;
FERARD, JF ;
VASSEUR, P .
CRYO-LETTERS, 1994, 15 (05) :269-278
[44]   Effects of Cryopreservation and Hypothermic Storage on Cell Viability and Enzyme Activity in Recombinant Encapsulated Cells Overexpressing Alpha-L-Iduronidase [J].
Mayer, Fabiana Quoos ;
Baldo, Guilherme ;
de Carvalho, Talita Giacomet ;
Lagranha, Valeska Lizzi ;
Giugliani, Roberto ;
Matte, Ursula .
ARTIFICIAL ORGANS, 2010, 34 (05) :434-439
[45]   Effects of oxidative stress on the changes of viability of Paeonia lactiflora seeds with different water content before and after cryopreservation [J].
Ji, Zhe ;
Guo, Jiayi ;
Huang, Jiaqi ;
Zhu, Qinglu ;
Ren, Ruifen .
CRYOBIOLOGY, 2024, 117
[46]   Effect of cryopreservation medium on post-thaw viability of equine testicular cells [J].
Ferrer, M. ;
Lutjemeier, B. ;
Lopez, Y. ;
Sanderson, M. ;
Lillich, J. ;
Weiss, M. .
THERIOGENOLOGY, 2008, 70 (03) :577-578
[47]   Effect of the desiccation tolerance and cryopreservation methods on the viability of Citrus limon L. Burm cv. Eureka seeds [J].
Orjuela-Palacio, Juliana M. ;
Graiver, Natalia ;
Victoria Santos, M. ;
Zaritzky, Noemi E. .
CRYOBIOLOGY, 2019, 89 :51-59
[48]   Viability of Red (Ribes rubrum L.) and Black (Ribes nigrum L.) Currant Cuttings in Field Conditions after Cryopreservation in Vapors of Liquid Nitrogen [J].
Verzhuk, Vladimir ;
Pavlov, Alexander ;
Novikova, Liubov Yu. ;
Filipenko, Galina .
AGRICULTURE-BASEL, 2020, 10 (10) :1-10
[49]   The survival of in vitro shoot tips of Garcinia mangostana L. after cryopreservation by vitrification [J].
Ibrahim, Sarah ;
Normah, M. N. .
PLANT GROWTH REGULATION, 2013, 70 (03) :237-246
[50]   Effects of Cryopreservation on the Transcriptome of Human Embryonic Stem Cells After Thawing and Culturing [J].
Wagh, Vilas ;
Meganathan, Kesavan ;
Jagtap, Smita ;
Gaspar, John Antonydas ;
Winkler, Johannes ;
Spitkovsky, Dimitry ;
Hescheler, Juergen ;
Sachinidis, Agapios .
STEM CELL REVIEWS AND REPORTS, 2011, 7 (03) :506-517
12345