DIFFERENTIATION OF BACTERIA USING PROTEIN PROFILES FROM MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

被引：173
作者：
CAIN, TC
LUBMAN, DM
WEBER, WJ
机构：
[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,DEPT CIVIL & ENVIRONM ENGN,ANN ARBOR,MI 48109
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 12期
关键词：
D O I：
10.1002/rcm.1290081224
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Matrix-assisted laser desorption/ionization time of-flight mass spectrometry is demonstrated as a means for rapidly differentiating bacteria based upon their water-soluble-protein profiles. The procedure involves release of cell contents by breaking fell membranes using a sonication method, and is performed with minimal sample preparation. Minimal purification of cell contents is required for the procedure. Protein profile signals obtained are found to be enhanced greatly by the use of a Nafion substrate.
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页码：1026 / 1030
页数：5
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