RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY IS LESS DISCRIMINANT THAN PULSED-FIELD GEL-ELECTROPHORESIS FOR TYPING STRAINS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS

被引：135

作者：

SAULNIER, P

BOURNEIX, C

PREVOST, G

ANDREMONT, A

机构：

[1] INST GUSTAVE ROUSSY,ECOL MICROBIENNE LAB,F-94805 VILLEJUIF,FRANCE

[2] FAC MED STRASBOURG,INST BACTERIOL,STRASBOURG,FRANCE

[3] FAC PHARM CHATENAY MALABRY,MICROBIOL LAB,F-92290 CHATENAY MALABRY,FRANCE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 04期

关键词：

D O I：

10.1128/JCM.31.4.982-985.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twenty-six strains of methicillin-resistant Staphylococcus aureus with different pulsed-field gel electrophoresis fingerprints were tested by random amplified polymorphic DNA assay with three primers, resulting in 15 to 20 different random amplified polymorphic DNA fingerprints. By summing the results for the three primers, the number of different fingerprints increased to 25, but two strains could not be differentiated. We conclude that pulsed-field gel electrophoresis remains the best method of typing methicillin-resistant S. aureus strains.

引用

页码：982 / 985

页数：4

共 34 条

[1] USE OF LOW-FREQUENCY-CLEAVAGE RESTRICTION ENDONUCLEASES FOR DNA ANALYSIS IN EPIDEMIOLOGICAL INVESTIGATIONS OF NOSOCOMIAL BACTERIAL-INFECTIONS [J].