CLINICAL-EVALUATION OF A NEW POLYMERASE CHAIN-REACTION ASSAY (AMPLICOR(TM) HCV) FOR DETECTION OF HEPATITIS-C VIRUS

被引：0

作者：

ZEUZEM, S ^{[1
]}

RUSTER, B ^{[1
]}

ROTH, WK ^{[1
]}

机构：

[1] GEORG SPEYER HAUS,CHEMOTHERAPEUT FORSCHUNGSINST,FRANKFURT,GERMANY

来源：

ZEITSCHRIFT FUR GASTROENTEROLOGIE | 1994年 / 32卷 / 06期

关键词：

REVERSE TRANSCRIPTION; PCR; HCV-GENOTYPES; RNA TRANSCRIPTS;

D O I：

暂无

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Direct detection of hepatitis C virus (HCV) by reverse transcription (RT) and polymerase chain reaction (PCR) has clinical impact on diagnosis and the assessment of anti-viral therapy. However, recent results of a quality control study on the detection of HCV-RNA by RT-PCR revealed inappropriate sensitivity and specificity in the majority of participating laboratories. In this study we evaluated the first standardized RT-PCR-assay (Amplicor(TM) HCV) for routine detection of hepatitis C virus in serum samples from patients with hepatitis C (n = 111 ), patients with resolved acute hepatitis C (n = 7) and controls (n = 101). The Amplicor(TM) HCV assay was convenient to handle, detected all genotypes of the hepatitis C virus commonly present in Europe (type 1, 2 and 3 according to Simmonds et al.) and had a lower detection limit of 10(2)-10(3) copies as assessed by quantifiable HCV-specific RNA templates. In the clinical evaluation the Amplicor(TM) HCV system reached a sensitivity of 100 % and a specificity of 97 %.

引用

页码：342 / 347

页数：6

共 19 条

[1]

ALTER HJ, 1989, NEW ENGL J MED, V321, P1484

[2] IMPORTANCE OF PRIMER SELECTION FOR THE DETECTION OF HEPATITIS-C VIRUS-RNA WITH THE POLYMERASE CHAIN-REACTION ASSAY [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :187-191

[3] SEQUENCE-ANALYSIS OF THE 5' NONCODING REGION OF HEPATITIS-C VIRUS [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4942-4946

[4] LOSS OF ANTIBODIES AGAINST HEPATITIS-C VIRUS IN HIV-SEROPOSITIVE INTRAVENOUS-DRUG-USERS [J].

CHAMOT, E ;

HIRSCHEL, B ;

WINTSCH, J ;

ROBERT, CF ;

GABRIEL, V ;

DEGLON, JJ ;

YERLY, S ;

PERRIN, L .

AIDS, 1990, 4 (12) :1275-1277

[5] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[6] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[7] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[8] DETECTION OF HEPATITIS-C VIRAL SEQUENCES IN BLOOD DONATIONS BY NESTED POLYMERASE CHAIN-REACTION AND PREDICTION OF INFECTIVITY [J].

GARSON, JA ;

TEDDER, RS ;

BRIGGS, M ;

TUKE, P ;

GLAZEBROOK, JA ;

TRUTE, A ;

PARKER, D ;

BARBARA, JAJ ;

CONTRERAS, M ;

ALOYSIUS, S .

LANCET, 1990, 335 (8703) :1419-1422

[9] AVOIDING FALSE POSITIVES WITH PCR [J].

KWOK, S ;

HIGUCHI, R .

NATURE, 1989, 339 (6221) :237-238

[10] REVERSE TRANSCRIPTION AND DNA AMPLIFICATION BY A THERMUS-THERMOPHILUS DNA-POLYMERASE [J].

MYERS, TW ;

GELFAND, DH .

BIOCHEMISTRY, 1991, 30 (31) :7661-7666

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