AN EFFICIENT AND EASY METHOD OF INFECTION OF MOSQUITO LARVAE FROM VIRUS-CONTAMINATED CELL-CULTURES

被引：17

作者：

BARREAU, C ^{[1
]}

JOUSSET, FX ^{[1
]}

CORNET, M ^{[1
]}

机构：

[1] INRA,RECH PATHOL COMPAREE STN,UNITE VIROL MOLEC,CNRS,UA 1184,F-30380 ST CHRISTOL ALES,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 49卷 / 02期

关键词：

EXPERIMENTAL INFECTION; MOSQUITO LARVA; VIRUS; CELL CULTURE;

D O I：

10.1016/0166-0934(94)90039-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for efficient infection of Aedes aegypti larvae by the Aedes albopictus densovirus, AaPV is described. It consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. After 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. Using this technique, up to 95% of first instar Ae. aegypti larvae were found infected by the AaPV.

引用

页码：153 / 156

页数：4

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