EXTRACELLULAR SERINE-PROTEASE SYNTHESIS BY MOSQUITO-PATHOGENIC STRAINS OF BACILLUS-SPHAERICUS

被引：9

作者：

DUMUSOIS, C

PRIEST, FG

机构：

[1] Department of Biological Sciences, Heriot-Watt University, Edinburgh

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 75卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1993.tb02796.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nine strains of Bacillus sphaericus toxic to mosquito larvae produced haloes of hydrolysis when cultured on casein-nutrient agar. In batch culture, protease synthesis by B. sphaericus BSE 18 occurred during exponential growth and was repressed by high concentrations of peptone. Extracellular protease from this bacterium showed optimal activity at about pH 10.2, was inhibited by phenylmethylsulphonyl chloride and chymostatin but not soybean trypsin inhibitor or EDTA. Hydrolysis of N-CBZ-glycine-nitrophenyl ester was consistent with the major enzyme being a serine protease.

引用

页码：416 / 419

页数：4

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