DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TRANSCRIPTS IN PERIPHERAL-BLOOD LYMPHOCYTES BY THE POLYMERASE CHAIN-REACTION

被引：19

作者：

BAGNARELLI, P

MENZO, S

MANZIN, A

VARALDO, PE

MONTRONI, M

GIACCA, M

CLEMENTI, M

机构：

[1] UNIV TRIESTE,INST MICROBIOL,VIA A FLEMING 22,I-34100 TRIESTE,ITALY

[2] UNIV ANCONA,INST MICROBIOL,I-60100 ANCONA,ITALY

[3] UNIV ANCONA,INST INTERNAL MED,I-60100 ANCONA,ITALY

[4] INT CTR GENET ENGN & BIOTECHNOL,TRIESTE,ITALY

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 32卷 / 01期

关键词：

POLYMERASE CHAIN REACTION; HIV-1; VIRAL TRANSCRIPT;

D O I：

10.1016/0166-0934(91)90182-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified application of the polymerase chain reaction (PCR) to the routine detection of human immunodeficiency virus type 1 (HIV-1) transcripts from peripheral lymphocytes of infected subjects is described. This technique is simpler than previously described assays and was shown to be highly sensitive after ethidium bromide staining of polyacrylamide gel electrophoresis of amplified material. The method can be used for the virologic evaluation of HIV-1-infected subjects, thus allowing early identification of seropositive patients with signs of active infection.

引用

收藏

页码：31 / 39

页数：9

相关论文

共 23 条

[1] ISOLATION OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) FROM PLASMA DURING PRIMARY HIV-INFECTION [J].

ALBERT, J ;

GAINES, H ;

SONNERBORG, A ;

NYSTROM, G ;

PEHRSON, PO ;

CHIODI, F ;

VONSYDOW, M ;

MOBERG, L ;

LIDMAN, K ;

CHRISTENSSON, B ;

ASJO, B ;

FENYO, EM .

JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (01) :67-73

[2] EFFICIENT ISOLATION OF HIV FROM PLASMA DURING DIFFERENT STAGES OF HIV INFECTION [J].

EHRNST, A ;

SONNERBORG, A ;

BERGDAHL, S ;

STRANNEGARD, O .

JOURNAL OF MEDICAL VIROLOGY, 1988, 26 (01) :23-32

[3] THE HUMAN IMMUNODEFICIENCY VIRUS - INFECTIVITY AND MECHANISMS OF PATHOGENESIS [J].

FAUCI, AS .

SCIENCE, 1988, 239 (4840) :617-622

[4] DETECTION OF LYMPHOCYTES EXPRESSING HUMAN LYMPHOTROPIC-T VIRUS TYPE-III IN LYMPH-NODES AND PERIPHERAL-BLOOD FROM INFECTED INDIVIDUALS BY INSITU HYBRIDIZATION [J].

HARPER, ME ;

MARSELLE, LM ;

GALLO, RC ;

WONGSTAAL, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (03) :772-776

[5]

HART C, 1988, LANCET, V2, P596

[6]

HIGUCHI R, 1989, AMPLIFICATIONS, V2, P1

[7]

HORSBURGH CR, 1989, LANCET, V2, P637

[8] HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 DETECTED IN ALL SEROPOSITIVE SYMPTOMATIC AND ASYMPTOMATIC INDIVIDUALS [J].

JACKSON, JB ;

KWOK, SY ;

SNINSKY, JJ ;

HOPSICKER, JS ;

SANNERUD, KJ ;

RHAME, FS ;

HENRY, K ;

SIMPSON, M ;

BALFOUR, HH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (01) :16-19

[9] RAPID AND SENSITIVE VIRAL CULTURE METHOD FOR HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 [J].

JACKSON, JB ;

COOMBS, RW ;

SANNERUD, K ;

RHAME, FS ;

BALFOUR, HH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (07) :1416-1418

[10] DIAGNOSIS OF CHRONIC MYELOID AND ACUTE LYMPHOCYTIC LEUKEMIAS BY DETECTION OF LEUKEMIA-SPECIFIC MESSENGER-RNA SEQUENCES AMPLIFIED INVITRO [J].

KAWASAKI, ES ;

CLARK, SS ;

COYNE, MY ;

SMITH, SD ;

CHAMPLIN, R ;

WITTE, ON ;

MCCORMICK, FP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5698-5702