DESCRIPTION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF PROTEIN TYROSINE KINASE

被引：39

作者：

LAZARO, I ^{[1
]}

GONZALEZ, M ^{[1
]}

ROY, G ^{[1
]}

VILLAR, LM ^{[1
]}

GONZALEZPORQUE, P ^{[1
]}

机构：

[1] HOSP RAMON & CAJAL,SERV INMUNOL,CARRETERA COLMENAR KM 9100,E-28034 MADRID,SPAIN

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90533-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A solid immunoassay for the detection of protein tyrosine kinases has been developed. It is based on the binding of the synthetic polypeptide poly(Glu·Na, Tyr) 4:1 to microELISA wells, where the phosphorylation reaction takes place in the presence of ATP and enzyme. The phosphorylated tyrosine residues produced in the reaction are finally detected, in the same well, by means of an ELISA using monoclonal antiphosphotyrosine antibody, peroxidase-labeled goat anti-mouse IgG antibody, and substrate. The amount of protein tyrosine kinase activity present in the sample is proportional to the color at 492 nm developed in each well. © 1991.

引用

页码：257 / 261

页数：5

共 7 条

[1]

BRAUN S, 1984, J BIOL CHEM, V259, P2051

[2]

CASNELLIE JE, 1983, J BIOL CHEM, V258, P738

[3] TYROSINE PHOSPHORYLATION OF CYTOSOLIC PROTEINS IN HUMAN ERYTHROCYTES [J].

CLARI, G ;

LIBERA, LD ;

MORET, V .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (03) :1378-1383

[4] CELL-LINES AND PERIPHERAL-BLOOD LEUKOCYTES DERIVED FROM INDIVIDUALS WITH CHRONIC MYELOGENOUS LEUKEMIA DISPLAY VIRTUALLY IDENTICAL PROTEINS PHOSPHORYLATED ON TYROSINE RESIDUES [J].

HUHN, RD ;

POSNER, MR ;

RAYTER, SI ;

FOULKES, JG ;

FRACKELTON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) :4408-4412

[5] A NONRADIOACTIVE DOT-BLOT ASSAY FOR PROTEIN TYROSINE KINASE-ACTIVITY [J].

RIJKSEN, G ;

VANOIRSCHOT, BA ;

STAAL, GEJ .

ANALYTICAL BIOCHEMISTRY, 1989, 182 (01) :98-102

[6]

ROSKOSKI R, 1983, METHOD ENZYMOL, V99, P3

[7]

ZIONCHECK TF, 1986, J BIOL CHEM, V261, P5637

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