IN-VIVO DETECTION OF SUPEROXIDE ANION PRODUCTION BY THE BRAIN USING A CYTOCHROME-C ELECTRODE

被引：106

作者：

FABIAN, RH

DEWITT, DS

KENT, TA

机构：

[1] UNIV TEXAS, MED BRANCH, DEPT ANESTHESIOL, GALVESTON, TX 77555 USA

[2] UNIV TEXAS, MED BRANCH, DEPT PSYCHIAT & BEHAV SCI, GALVESTON, TX 77555 USA

[3] UNIV TEXAS, MED BRANCH, DEPT PHARMACOL & TOXICOL, GALVESTON, TX 77555 USA

[4] UNIV TEXAS, MED BRANCH, INST MARINE BIOMED, GALVESTON, TX 77555 USA

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 1995年 / 15卷 / 02期

关键词：

BRAIN; CYTOCHROME C; ELECTRODE; SUPEROXIDE ANION;

D O I：

10.1038/jcbfm.1995.30

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A cytochrome c-coated platinized carbon electrode was utilized to detect superoxide generated by the brain during hypoxia/hypercarbia, focal ischemia, and reperfusion and following fluid percussion brain injury with and without hemorrhagic hypotension and reperfusion in the rat. All three of these forms of brain injury were associated with an increase in the superoxide signal. The cytochrome c electrode proved to be sensitive and responsive enough for minute-by-minute measurement of superoxide generation by brain tissue.

引用

页码：242 / 247

页数：6