SEPARATION OF SYNTHETIC PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES FROM THEIR OXYGENATED (PHOSPHODIESTER) DEFECT SPECIES BY STRONG-ANION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：70

作者：

BERGOT, BJ ^{[1
]}

EGAN, W ^{[1
]}

机构：

[1] US FDA,CTR BIOL EVALUAT & RES,BIOPHYS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 599卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85456-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described which separates synthetic deoxynucleotide phosphorothioates from their oxygenated (phosphodiester, "P=O") defect species by strong-anion-exchange chromatography, using novel "soft-base" anionic eluents. The method enables the qualitative and quantitative assessment of P=O content in phosphorothioate DNA, and represents a rapid and sample-conserving alternative to P-31-NMR.

引用

页码：35 / 42

页数：8

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