SEPARATION OF SYNTHETIC PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES FROM THEIR OXYGENATED (PHOSPHODIESTER) DEFECT SPECIES BY STRONG-ANION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：68
作者：
BERGOT, BJ [1 ]
EGAN, W [1 ]
机构：
[1] US FDA,CTR BIOL EVALUAT & RES,BIOPHYS LAB,BETHESDA,MD 20892
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 599卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)85456-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method is described which separates synthetic deoxynucleotide phosphorothioates from their oxygenated (phosphodiester, "P=O") defect species by strong-anion-exchange chromatography, using novel "soft-base" anionic eluents. The method enables the qualitative and quantitative assessment of P=O content in phosphorothioate DNA, and represents a rapid and sample-conserving alternative to P-31-NMR.
引用
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页码：35 / 42
页数：8
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