INHIBITORY EFFECT OF MYCOPLASMA-RELEASED ARGINASE - ACTIVITY IN MIXED-LYMPHOCYTE AND TUMOR-CELL CULTURES

被引：7

作者：

CLAESSON, MH ^{[1
]}

TSCHERNING, T ^{[1
]}

NISSEN, MH ^{[1
]}

LIND, K ^{[1
]}

机构：

[1] STATENS SERUM INST,DK-2300 COPENHAGEN,DENMARK

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1990年 / 32卷 / 06期

关键词：

D O I：

10.1111/j.1365-3083.1990.tb03204.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Non‐fermenting mycoplasma species deplete culture media for arginine through arginase activity linked to their arginine deiminase pathway, resulting in proliferation arrest and cell death in mycoplasma‐contaminated cell cultures. The presence of only 2‐3 Mycoplasma (M.) arginini‐contaminated T cells in a one‐way allogeneic mixed‐lymphocyte culture (MLC) significantly inhibits development of cytotoxic T‐cell activity. Likewise, strong degrees of inhibition are observed after addition of nanogram doses of M. arginini extracts (MAE) to MLC or cell proliferation cultures. M. arginini‐induced cell inhibition can be reversed by addition of excess arginine to the culture medium. Antisera raised against non‐fermenting, but not against fermenting, mycoplasma species block the inhibitory effect of MAE. SDS‐PAGE separation of MAE disclosed a broad band at 60 kDa which contained arginase activity when assayed in MLC and cell proliferation culture. SDS PAGE followed by western blotting and reaction with antisera raised against non‐fermenting mycoplasma species demonstrated a band at 43 kDa common for these micro‐organisms. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：623 / 630

页数：8

共 20 条

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