EXPRESSION OF RESISTANCE TO BARLEY STRIPE MOSAIC-VIRUS IN BARLEY AND OAT PROTOPLASTS

被引:9
作者
ZHENG, Y [1 ]
EDWARDS, MC [1 ]
机构
[1] USDA ARS, CEREAL CROPS RES UNIT,NO CROP SCI LAB,POB 5677, STATE UNIV STN, FARGO, ND 58105 USA
关键词
D O I
10.1099/0022-1317-71-8-1865
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Mesophyll protoplasts from both susceptible and resistant hosts were inoculated with RNA purified from barley stripe mosaic virus (BSMV) strains CV52 and CV42 using the polyethylene glycol (PEG) method. Protoplasts derived from the susceptible Hordeum vulgare L. cv. Black Hulless were susceptible to both BSMV strains, as indicated by fluorescein isothiocyanate staining and ELISA. More than 80% of protoplasts derived from an oat cultivar resistant to CV52, but not to CV42, were readily infected by either CV52 or CV42. Protoplasts from 10 barley lines resistant to CV42 remained resistant to CV42, although a limited number of protoplasts were infected. Functional resistance in the cultured barley protoplasts, but not in those from oat plants, suggests that resistance in these barley lines may be the result of restriction of replication, whereas resistance in oat plants is more likely due to restriction of cell-to-cell movement.
引用
收藏
页码:1865 / 1868
页数:4
相关论文
共 25 条
[1]   INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC VIRUS RIBONUCLEIC ACID [J].
AOKI, S ;
TAKEBE, I .
VIROLOGY, 1969, 39 (03) :439-&
[2]   EXPRESSION OF GENES FOR RESISTANCE TO POTATO VIRUS-Y IN POTATO PLANTS AND PROTOPLASTS [J].
BARKER, H ;
HARRISON, BD .
ANNALS OF APPLIED BIOLOGY, 1984, 105 (03) :539-545
[3]  
BEIER H, 1977, PHYTOPATHOLOGY, V67, P917, DOI 10.1094/Phyto-67-917
[4]   REPLICATION OF COWPEA MOSAIC-VIRUS IN PROTOPLASTS ISOLATED FROM IMMUNE LINES OF COWPEAS [J].
BEIER, H ;
BRUENING, G ;
RUSSELL, ML ;
TUCKER, CL .
VIROLOGY, 1979, 95 (01) :165-175
[5]   CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES [J].
CLARK, MF ;
ADAMS, AN .
JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) :475-483
[6]  
COUTTS RHA, 1977, FEMS MICROBIOL LETT, V1, P121, DOI 10.1111/j.1574-6968.1977.tb00595.x
[7]   GENETIC-ANALYSIS OF BARLEY STRIPE MOSAIC-VIRUS [J].
EDWARDS, MC ;
TIMIAN, RG .
PHYTOPATHOLOGY, 1986, 76 (03) :360-365
[8]   EXPRESSION OF RESISTANCE TO POTATO VIRUS-X IN ISOLATED POTATO PROTOPLASTS [J].
FOXE, MJ ;
PRAKASH, J .
ARCHIVES OF VIROLOGY, 1986, 88 (3-4) :167-174
[9]   CUCUMBER MOSAIC-VIRUS REPLICATION IN COWPEA PROTOPLASTS - TIME COURSE OF VIRUS, COAT PROTEIN AND RNA-SYNTHESIS [J].
GONDA, TJ ;
SYMONS, RH .
JOURNAL OF GENERAL VIROLOGY, 1979, 45 (DEC) :723-736
[10]  
HUGHES BG, 1978, BIOCHEM PHYSIOL PFL, V172, P67