SEPARATION OF ALPHA-1, ALPHA-2 AND ALPHA-3 SUBUNITS OF THE GABA(A)-BENZODIAZEPINE RECEPTOR COMPLEX BY IMMUNOAFFINITY CHROMATOGRAPHY

被引：58

作者：

ZEZULA, J ^{[1
]}

FUCHS, K ^{[1
]}

SIEGHART, W ^{[1
]}

机构：

[1] UNIV VIENNA,PSYCHIAT KLIN,DEPT BIOCHEM PSYCHIAT,WAHRINGER GURTEL 18-20,A-1090 VIENNA,AUSTRIA

来源：

BRAIN RESEARCH | 1991年 / 563卷 / 1-2期

关键词：

ANTIBODY; GAMMA-AMINOBUTYRIC ACID-BENZODIAZEPINE RECEPTOR; SUBUNIT; HETEROGENEITY;

D O I：

10.1016/0006-8993(91)91556-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Polyclonal antibodies raised to synthetic amino acid sequences of the rat gamma-aminobutyric acid (GABA(A)) receptor alpha-1-, alpha-2- and alpha-3-subunits selectively recognized single proteins with apparent molecular weight 51 kDa (P51), 53 kDa (P53) and 59 kDa (P59), respectively, in GABA(A) receptor preparations affinity purified from the brains of 5-10-day-old rats. The antibodies were coupled to Affigel 10, and the resulting immunoaffinity columns were used to isolate these proteins from affinity purified GABA(A) receptors.

引用

页码：325 / 328

页数：4

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