ULTRASTRUCTURAL CHARACTERIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GAG-CONTAINING PARTICLES ASSEMBLED IN A RECOMBINANT ADENOVIRUS VECTOR SYSTEM

被引：29
作者：
VERNON, SK
MURTHY, S
WILHELM, J
CHANDA, PK
KALYAN, N
LEE, SG
HUNG, PP
机构：
[1] Biotechnology and, Microbiology Division, Wyeth-Ayerst Research, Philadelphia, PA 19101
来源：
JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷
关键词：
D O I：
10.1099/0022-1317-72-6-1243
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 gag and pro genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.
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页码：1243 / 1251
页数：9
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