PROPERTIES OF A NOVEL NITRIC OXIDE-STIMULATED ADP-RIBOSYLTRANSFERASE

被引：58

作者：

BRUNE, B ^{[1
]}

LAPETINA, EG ^{[1
]}

机构：

[1] BURROUGHS WELLCOME CO,DIV CELL BIOL,RES TRIANGLE PK,NC 27709

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 279卷 / 02期

关键词：

D O I：

10.1016/0003-9861(90)90493-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel ADP-ribosyltransferase is present in the cytosolic fraction of various cells. The kinetic behavior and physical properties of this enzyme's activity are clearly distinguished from other known cytosolic ADP-ribosyltransferases. Agents that release nitric oxide, such as sodium nitroprusside, greatly stimulated this activity, although this effect was dependent on the presence of intact thiol groups. Dithiothreitol, reduced glutathione, or cysteine was needed for activation of the enzyme, while N-ethylmaleimide inhibited enzyme activity. High concentrations of phosphate had a slight stimulatory effect, while high concentrations of sodium chloride and thiocyanate were inhibitory. ATP also inhibited this activity. This cytosolic ADP-ribosyltransferase is clearly distinguished from other known and characterized cytosolic transferases. Its activation by biologically active nitric oxide suggests an important role for this enzymatic activity. © 1990.

引用

页码：286 / 290

页数：5

共 28 条

[1] DIFFERENT TYPES OF ADP-RIBOSE PROTEIN BONDS FORMED BY BOTULINUM C2 TOXIN, BOTULINUM ADP-RIBOSYLTRANSFERASE C-3 AND PERTUSSIS TOXIN [J].

AKTORIES, K ;

JUST, I ;

ROSENTHAL, W .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (01) :361-367

[2]

Baenziger N L, 1974, Methods Enzymol, V31, P149

[3] ADP-RIBOSYLATION OF ISOLATED RAT ISLETS OF LANGERHANS [J].

BERNOFSKY, C ;

AMAMOO, DG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 118 (02) :663-668

[4]

BRUNE B, 1989, J BIOL CHEM, V264, P8455

[5] (ADP-RIBOSE)N PARTICIPATES IN DNA EXCISION REPAIR [J].

DURKACZ, BW ;

OMIDIJI, O ;

GRAY, DA ;

SHALL, S .

NATURE, 1980, 283 (5747) :593-596

[6] THE OBLIGATORY ROLE OF ENDOTHELIAL-CELLS IN THE RELAXATION OF ARTERIAL SMOOTH-MUSCLE BY ACETYLCHOLINE [J].

FURCHGOTT, RF ;

ZAWADZKI, JV .

NATURE, 1980, 288 (5789) :373-376

[7] POLY(ADP-RIBOSE) AND ADP-RIBOSYLATION OF PROTEINS [J].

HAYAISHI, O ;

UEDA, K .

ANNUAL REVIEW OF BIOCHEMISTRY, 1977, 46 :95-116

[8] ROLE OF DNA BREAKS AND ADP-RIBOSYL TRANSFERASE-ACTIVITY IN EUKARYOTIC DIFFERENTIATION DEMONSTRATED IN HUMAN-LYMPHOCYTES [J].

JOHNSTONE, AP ;

WILLIAMS, GT .

NATURE, 1982, 300 (5890) :368-370

[9] CELL CYCLE-DEPENDENT INTERVENTION BY BENZAMIDE OF CARCINOGEN-INDUCED NEOPLASTIC TRANSFORMATION AND INVITRO POLY(ADP-RIBOSYL)ATION OF NUCLEAR PROTEINS IN HUMAN-FIBROBLASTS [J].

KUN, E ;

KIRSTEN, E ;

MILO, GE ;

KURIAN, P ;

KUMARI, HL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23) :7219-7223

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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