INFLUENCE OF BIOLOGICAL PARAMETERS AND GENE-TRANSFER TECHNIQUE ON TRANSFORMATION OF FUSARIUM-OXYSPORUM

被引：26

作者：

LANGIN, T ^{[1
]}

DABOUSSI, MJ ^{[1
]}

GERLINGER, C ^{[1
]}

BRYGOO, Y ^{[1
]}

机构：

[1] UNIV PARIS 11,CRYPTOGAMIE LAB,BAT 400,F-91405 ORSAY,FRANCE

来源：

CURRENT GENETICS | 1990年 / 17卷 / 04期

关键词：

Electroporation; Fungal transformation; Fusarium oxysporum; Nitrate reductase;

D O I：

10.1007/BF00314878

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An improved method for PEG-mediated transformation of the fungal pathogen Fusarium oxysporum was developed which led to at least a 10-fold increase in transformation frequency. This improvement was gained through the analysis of biological factors, viz., protoplast origin, plasmid modifications and protoplast/plasmid ratio. Elecroporation, a recently developed method for introducing DNA into many cell types, was successfully applied. It gave similar transformation efficiencies to those obtained with the chemical method, and thus appeared a valuable alternative. Qualitative features such as integration events were also analysed. Molecular analysis of transformants revealed that a single copy of plasmid DNA was preferentially integrated by electroporation. The respective advantages of the two DNA transfer methods are discussed. © 1990 Springer-Verlag.

引用

页码：313 / 319

页数：7

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