A SIMPLE HPLC METHOD FOR THE DETERMINATION OF S-ADENOSYLMETHIONINE AND S-ADENOSYLHOMOCYSTEINE IN RAT-TISSUES - THE EFFECT OF VITAMIN-B-6 DEFICIENCY ON THESE CONCENTRATIONS IN RAT-LIVER

被引：110
作者：
SHE, QB
NAGAO, I
HAYAKAWA, T
TSUGE, H
机构：
[1] GIFU UNIV, FAC AGR, DEPT FOOD SCI, GIFU 50111, JAPAN
[2] GIFU UNIV, UNITED GRAD SCH AGR SCI, GIFU 50111, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期
关键词：
D O I：
10.1006/bbrc.1994.2871
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method using isocratic HPLC with ultraviolet detection was established for the simultaneous measurement of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in rat tissues. The method provides rapid resolution of both compounds in a single run by direct injection of the perchloric acid extract of tissue, so that sampling procedures and analytical errors can be reduced in. determining the SAM/SAH ratio, a measure of transmethylation reactions. The assay has a detection limit of 25 pmol and is linear in the range 50-2000 pmol. This procedure was applied to determine the change of SAM/SAH ratio in liver caused by vitamin B6 deficiency. The marked reduction of the SAM/SAH ratios in vitamin B6-deficient rats were due to a drastic rise in SAH concentration with a concurrent striking decrease in SAM concentration. (C) 1994 Academic Press, Inc.
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页码：1748 / 1754
页数：7
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