DETECTION OF CORTICOSTEROIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY USING A PARTICLE-BEAM INTERFACE

被引：15

作者：

STANLEY, SMR ^{[1
]}

WILHELMI, BS ^{[1
]}

RODGERS, JP ^{[1
]}

GUTHRIE, A ^{[1
]}

机构：

[1] UNIV PRETORIA,FAC VET SCI,EQUINE RES CTR,ONDERSTEPOORT 0110,SOUTH AFRICA

来源：

BIOLOGICAL MASS SPECTROMETRY | 1994年 / 23卷 / 08期

关键词：

D O I：

10.1002/bms.1200230805

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A normal-phase high-performance liquid chromatographic/particle beam mass spectrometric (negative chemical ionization) method has been developed for the detection of synthetic corticosteroids (SC). Characteristic and reproducible full-scan spectra were obtained for the SC analysed. The limit of detection using selected ion monitoring for the molecular anion of beclomethasone was 500 pg. Urine from an equine to which 9 alpha-fluoroprednisolone acetate had been administered was extracted by immunoaffinity chromatography and analysed by high-performance liquid chromatography-ultraviolet detection/particle beam mass spectrometry. Full-scan spectral data from these extracts allowed positive identification of 9 alpha-fluoroprednisolone (isoflupredone) in the equine urine up to 96 h post administration.

引用

页码：483 / 491

页数：9

共 12 条

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