THE EFFECT OF D-PENICILLAMINE ON METALLOTHIONEIN MESSENGER-RNA LEVELS AND COPPER DISTRIBUTION IN MOUSE HEPATOCYTES

被引：12

作者：

MCARDLE, HJ ^{[1
]}

KYRIAKOU, P ^{[1
]}

GRIMES, A ^{[1
]}

MERCER, JFB ^{[1
]}

DANKS, DM ^{[1
]}

机构：

[1] ROYAL CHILDRENS HOSP,MURDOCH INST,SCOBIE & CLAIRE MACKINNON TRACE ELEMENT RES GRP,PARKVILLE,VIC 3052,AUSTRALIA

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1990年 / 75卷 / 03期

关键词：

Copper transport - Copper uptake - Wilson's disease - Diamsar - Liver - Metallothionein 1 - Copper binding;

D O I：

10.1016/0009-2797(90)90074-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Penicillamine increases the levels of metallothionein (MT) mRNA in a time and concentration dependent manner without altering either the rate of copper uptake or the amount of copper within the cell. The effect is dependent on the presence of intracellular copper, however, since depletion of copper by chelators blocks the effect, and does not alter the ability of dexamethasone to stimulate mRNA production. Penicillamine did not alter the distribution of 64Cu in the hepatocytes, as measured by fast protein liquid chromatography (FPLC), although the pattern may be affected by the amount of MT present. The data indicates that penicillamine removes copper from some intermediary ligand, thereby making it available to induce metallothionein. It is possible that this is part of the therapeutic action of the chelator in the treatment of Wilson's disease. © 1990.

引用

页码：315 / 324

页数：10