EPILIGRIN, A COMPONENT OF EPITHELIAL BASEMENT-MEMBRANES, IS AN ADHESIVE LIGAND FOR ALPHA-3-BETA-1 POSITIVE T-LYMPHOCYTES

被引:161
|
作者
WAYNER, EA
GIL, SG
MURPHY, GF
WILKE, MS
CARTER, WG
机构
[1] UNIV MINNESOTA,CTR BIOMED ENGN,MINNEAPOLIS,MN 55455
[2] UNIV PENN,DEPT DERMATOL,PHILADELPHIA,PA 19104
[3] FRED HUTCHINSON CANC RES CTR,DEPT CELL BIOL,SEATTLE,WA 98104
来源
JOURNAL OF CELL BIOLOGY | 1993年 / 121卷 / 05期
关键词
D O I
10.1083/jcb.121.5.1141
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cutaneous T cell lymphomas (CTCL), typified by mycosis fungoides, and several chronic T cell mediated dermatoses are characterized by the migration of T lymphocytes into the epidermis (epidermotropism). Alternatively, other types of cutaneous inflammation (malignant cutaneous B cell lymphoma, CBCL, or lymphocytoma cutis, non-malignant T or B cell type) do not show evidence of epidermotropism. This suggests that certain T lymphocyte subpopulations are able to interact with and penetrate the epidermal basement membrane. We show here that T lymphocytes derived from patients with CTCL (HUT 78 or HUT 102 cells), adhere to the detergent-insoluble extracellular matrix prepared from cultured basal keratinocytes (HFK ECM). HUT cell adhesion to HFK ECM was inhibitable with monoclonal antibodies (mAbs) directed to the alpha3 (P1B5) or beta1 (P4C10) integrin receptors, and could be up-regulated by an activating anti-beta1 mAb (P4G11). An inhibitory mAb, P3H9-2, raised against keratinocytes identified epiligrin as the ligand for alpha3beta1 positive T cells in HFK ECM. Interestingly, two lymphocyte populations could be clearly distinguished relative to expression of alpha3beta1 by flow cytometry analysis. Lymphokine activated killer cells, alloreactive cytotoxic T cells and T cells derived from patients with CTCL expressed high levels of alpha3beta1 (alpha3beta1 high). Non-adherent peripheral blood mononuclear cells, acute T or B lymphocytic leukemias, or non-cutaneous T or B lymphocyte cell lines expressed low levels of alpha3beta1 (alpha3beta1low). Resting PBL or alpha3beta1low T or B cell lines did not adhere to HFK ECM or purified epiligrin. However, adhesion to epiligrin could be up-regulated by mAbs which activate the beta1 subunit indicating that alpha3beta1 activity is a function of expression and affinity. In skin derived from patients with graft-vs.-host (GVH) disease, experimentally induced delayed hypersensitivity reactions, and CTCL, the infiltrating T cells could be stained with mAbs to alpha3 or beta1 and were localized in close proximity to the epiligrin-containing basement membrane. Infiltrating lymphocytes in malignant cutaneous B disease (CBCL) did not express aLPHA3beta1 by immunohistochemical techniques and did not associate with the epidermal basement membrane. The present findings clearly define a function for alpha3beta1 in T cells and strongly suggest that alpha3beta1 interaction with epiligrin may be involved in the pathogenesis of cutaneous inflammation.
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页码:1141 / 1152
页数:12
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