THE CONTROL OF CELLULAR-SHAPE AND MOTILITY - MG-2+ AND TROPOMYOSIN REGULATE THE FORMATION AND THE DISSOCIATION OF MICROFILAMENT BUNDLES

被引：8
作者：
GRAZI, E
CUNEO, P
CATALDI, A
机构：
[1] Istituto di Chimica Biologica, Universita di Ferrara, 44100 Ferrara
来源：
BIOCHEMICAL JOURNAL | 1992年 / 288卷
关键词：
D O I：
10.1042/bj2880727
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
At pH 7.14 and 37-degrees-C, in 7.2 % (w/v) poly(ethylene glycol) 6000, tropomyosin-regulated actin filaments are converted into filament bundles by increasing the free Mg2+ concentration to 1.7-2.0 mm. When free Mg2+ concentration is decreased below 1.7 mM, bundles dissociate back into tropomyosin-regulated actin filaments. Pure actin filaments are insensitive to this mechanism of control and are found as filament bundles in all the range of free Mg2+ concentrations tested (1.37-2.2 mm). The mechanism of regulation described above is likely to operate in the cell, where the concentration of free Mg2+ is linked to the energy charge of the adenine nucleotide system.
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页码：727 / 732
页数：6
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