RESOLUTION OF THE CONFORMATIONAL DISTRIBUTION AND DYNAMICS OF A FLEXIBLE MOLECULE USING FREQUENCY-DOMAIN FLUOROMETRY

被引：25
|
作者：
LAKOWICZ, JR [1 ]
KUSBA, J [1 ]
WICZK, W [1 ]
GRYCZYNSKI, I [1 ]
SZMACINSKI, H [1 ]
JOHNSON, ML [1 ]
机构：
[1] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908
来源：
BIOPHYSICAL CHEMISTRY | 1991年 / 39卷 / 01期
关键词：
FLUORESCENCE; MOLECULAR DYNAMICS; CONFORMATIONAL ANALYSIS; RESONANCE ENERGY TRANSFER; FREQUENCY-DOMAIN FLUOROMETRY;
D O I：
10.1016/0301-4622(91)85008-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to meaurements of structural fluctuations of biological macromolecules.
引用
收藏
页码：79 / 84
页数：6
相关论文
共 50 条
  • [1] RESOLUTION OF AN EXCITED-STATE REACTION USING FREQUENCY-DOMAIN FLUOROMETRY
    LAKOWICZ, JR
    CHEREK, H
    CHEMICAL PHYSICS LETTERS, 1985, 122 (04) : 380 - 384
  • [2] RESOLUTION OF A DISTRIBUTION OF DISTANCES BY FLUORESCENCE ENERGY-TRANSFER AND FREQUENCY-DOMAIN FLUOROMETRY
    LAKOWICZ, JR
    JOHNSON, ML
    WICZK, W
    BHAT, A
    STEINER, RF
    CHEMICAL PHYSICS LETTERS, 1987, 138 (06) : 587 - 593
  • [3] MULTIPLE CONFORMATIONAL STATES IN MYOGLOBIN REVEALED BY FREQUENCY-DOMAIN FLUOROMETRY
    BISMUTO, E
    IRACE, G
    GRATTON, E
    BIOCHEMISTRY, 1989, 28 (04) : 1508 - 1512
  • [4] DISTRIBUTION OF DISTANCES IN MACROMOLECULES FROM FREQUENCY-DOMAIN FLUOROMETRY
    LAKOWICZ, JR
    MALIWAL, B
    KEATING, S
    BIOPHYSICAL JOURNAL, 1985, 47 (02) : A210 - A210
  • [5] Frequency-domain fluorometry.
    Nakaema, MKK
    Sanches, R
    QUIMICA NOVA, 1999, 22 (03): : 412 - 416
  • [6] RESOLUTION OF SUBNANOSECOND ANISOTROPY DECAYS OF TRYPTOPHAN PROTEIN FLUORESCENCE USING FREQUENCY-DOMAIN FLUOROMETRY
    LAKOWICZ, JR
    GRYCZYNSKI, I
    CHEREK, H
    MALIWAL, BP
    BIOPHYSICAL JOURNAL, 1986, 49 (02) : A494 - A494
  • [7] RESOLUTION OF SUBNANOSECOND ANISOTROPY DECAYS OF TRYPTOPHAN PROTEIN FLUORESCENCE USING FREQUENCY-DOMAIN FLUOROMETRY
    LAKOWICZ, JR
    GRYCZYNSKI, I
    CHEREK, H
    MALIWAL, BP
    FEDERATION PROCEEDINGS, 1986, 45 (06) : 1606 - 1606
  • [8] DETECTION OF 3 ROTATIONAL CORRELATION TIMES FOR A RIGID ASYMMETRIC MOLECULE USING FREQUENCY-DOMAIN FLUOROMETRY
    GRYCZYNSKI, I
    CHEREK, H
    LAKOWICZ, JR
    BIOPHYSICAL CHEMISTRY, 1988, 30 (03) : 271 - 277
  • [9] DETECTOR NONLINEARITY IN FREQUENCY-DOMAIN FLUOROMETRY
    WIRTH, MJ
    BURBAGE, JD
    ZULLI, SL
    APPLIED OPTICS, 1993, 32 (06) : 976 - 980
  • [10] Background suppression in frequency-domain fluorometry
    Lakowicz, JR
    Gryczynski, I
    Gryczynski, Z
    Johnson, ML
    ANALYTICAL BIOCHEMISTRY, 2000, 277 (01) : 74 - 85
12345