OVARIAN INTRABURSAL ADMINISTRATION OF INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN INHIBITS FOLLICLE RUPTURE IN GONADOTROPIN-TREATED IMMATURE FEMALE RATS

被引:58
作者
BICSAK, TA
LING, N
DEPAOLO, LV
机构
[1] Whittier Inst. for Diabetes, La Jolla, CA 92037
关键词
D O I
10.1095/biolreprod44.4.599
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effects of an insulin-like growth factor-binding protein (IGF-BP) on rat follicular function were examined by using the technique of ovarian intrabursal (IB) injection. Immature female rats were injected with 15 IU of eCG followed immediately with IB injections of 4-mu-g IGF-BP3 (right ovary). and vehicle (left ovary). Forty-eight hours later, the same animals were either killed (eCG-treated group) or injected with 1-mu-g of hCG as an ovulatory stimulus. These animals were killed 24 h later (eCG/hCG-treated group). Intrabursal administration of IGF-BP3 inhibited ovulations in the eCG/hCG-treated rats by 55% when compared with the contralateral vehicle-treated ovary (p = 0.01). Examination of the ovaries exposed to IGF-BP3 revealed the presence of unruptured follicles containing a matured oocyte and a disintegrated basement membrane, in addition to normal follicles and corpora lutea. In contrast, IB injection of IGF-BP3 had no effect on ovarian weights or circulating estradiol concentrations in the eCG-treated animals, and the ovaries appeared to be morphologically normal. Ligand blotting experiments using [I-125]-labeled insulin-like growth factor I revealed that granulosa cells obtained from both untreated and eCG-treated rats synthesized and secreted two IGF-BPs of M(r) 35 000 and 30 000. Equine chorionic gonadotropin treatment reduced the amount of the 30 000 M(r) form of IGF-BP. These data suggest that locally produced ovarian IGF-BPs may modulate follicle functions in vivo.
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页码:599 / 603
页数:5
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