ESTERIFICATION OF 2-METHYLALKANOIC ACIDS CATALYZED BY LIPASE FROM CANDIDA-RUGOSA - ENANTIOSELECTIVITY AS A FUNCTION OF WATER ACTIVITY AND ALCOHOL CHAIN-LENGTH

被引：25

作者：

BERGLUND, P ^{[1
]}

VORDE, C ^{[1
]}

HOGBERG, HE ^{[1
]}

机构：

[1] MID SWEDEN UNIV, DEPT CHEM, S-85170 SUNDSVALL, SWEDEN

来源：

BIOCATALYSIS | 1994年 / 9卷 / 1-4期

关键词：

CANDIDA-RUGOSA LIPASE; ESTERIFICATION; ENANTIOSELECTIVITY; WATER ACTIVITY; IMMOBILIZATION; ENZYME RECOVERY;

D O I：

10.3109/10242429408992114

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Esterifications catalysed by immobilised lipase from Candida rugosa CRL) in cyclohexane at constant water activity (a(w) = 0.76) were studied using 2-methyl substituted octa-, nona- or decanoic acids and n-alcohols of varying chain length as substrates. The importance of controlling the water activity and choosing the right alcohol for obtaining maximum enantioselectivity is demonstrated. The immobilised lipase was easily recovered without loss of activity and enantioselectivity.

引用

页码：123 / 130

页数：8

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