PURIFICATION OF RECOMBINANT PROTEIN-A BY AQUEOUS 2-PHASE EXTRACTION INTEGRATED WITH AFFINITY PRECIPITATION

被引：67

作者：

KAMIHIRA, M ^{[1
]}

KAUL, R ^{[1
]}

MATTIASSON, B ^{[1
]}

机构：

[1] UNIV LUND,DEPT BIOTECHNOL,CTR CHEM,S-22101 LUND,SWEDEN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 11期

关键词：

AQUEOUS 2-PHASE EXTRACTION; AFFINITY PRECIPITATION; EUDRAGIT; PROTEIN-A;

D O I：

10.1002/bit.260401112

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aqueous two-phase extraction incorporated affinity precipitation was examined as a technique for protein purification. An enteric coating polymer, Eudragit S100, was employed as a ligand carrier. Eudragit was specifically partitioned to the top phase in the aqueous two-phase systems. For application of this method to purification of recombinant protein A using human IgG coupled to Eudragit in an aqueous two-phase system, 80% of protein A added was recovered with 81% purity. The purity was enhanced 26-fold by this method. The IgG-Eudragit could be used repeatedly for the purification process. This separation method should be applicable to industrial-scale purification as a new purification procedure combining the advantages and compensating for the disadvantages of the aqueous two-phase method and affinity precipitation method.

引用

页码：1381 / 1387

页数：7

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