CHARACTERIZATION OF THE VPG DSRNA LINKAGE OF INFECTIOUS PANCREATIC NECROSIS VIRUS

被引：60

作者：

CALVERT, JG ^{[1
]}

NAGY, E ^{[1
]}

SOLER, M ^{[1
]}

DOBOS, P ^{[1
]}

机构：

[1] UNIV GUELPH,COLL BIOL SCI,DEPT MICROBIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷

关键词：

D O I：

10.1099/0022-1317-72-10-2563

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of I-125-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VPI (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5' GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.

引用

页码：2563 / 2567

页数：5

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