NEW NORFLOXACIN RESISTANCE GENE IN PSEUDOMONAS-AERUGINOSA PAO

被引：121
作者：
FUKUDA, H
HOSAKA, M
HIRAI, K
IYOBE, S
机构：
[1] KYORIN PHARMACEUT CO LTD,CENT RES LABS,NOGI,TOCHIGI,JAPAN
[2] GUNMA UNIV,SCH MED,DEPT MICROBIOL,MAEBASHI,GUNMA 371,JAPAN
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1990年 / 34卷 / 09期
关键词：
D O I：
10.1128/AAC.34.9.1757
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new type of norfloxacin-resistant mutant of Pseudomonas aeruginosa PAO was isolated. This mutant showed cross resistance to imipenem and chloramphenicol and hypersusceptibility to β-lactam and aminoglycoside antibodies. The new norfloxacin resistance gene nfxC was mapped near catA (46 min) on the PAO chromosome. Norfloxacin accumulation was decreased in the nfxC mutant; furthermore, the rate of imipenem diffusion through the outer membrane of the nfxC mutant was lower than that of the parent strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins showed a decrease of a 46-kilodalton protein and an increase of a 50-kilodalton in the nfxC mutant. We conclude that nfxC is a new norfloxacin resistance gene that affects outer membrane permeability to quinolones and other antimicrobial agents.
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页码：1757 / 1761
页数：5
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