INTRAMOLECULAR DNA TRIPLEXES - UNUSUAL SEQUENCE REQUIREMENTS AND INFLUENCE ON DNA POLYMERIZATION

被引：92
|
作者：
DAYN, A [1 ]
SAMADASHWILY, GM [1 ]
MIRKIN, SM [1 ]
机构：
[1] UNIV ILLINOIS,DEPT GENET,CHICAGO,IL 60612
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 23期
关键词：
H-ASTERISK-DNA; DNA POLYMERASE; TERMINATION;
D O I：
10.1073/pnas.89.23.11406
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Homopurine-homopyrimidine mirror repeats are known to form intramolecular DNA triplexes in vitro. By probing with chemical agents specific for unusual DNA conformations, we have now demonstrated the formation of intramolecular triplexes consisting of G.G.C and T.A.T base triplets by DNA sequences that are neither homopurine-homopyrimidine nor mirror repeats. This finding significantly enlarges the number of sequences that could form DNA triplexes. The observed triplexes are stable under the conditions that are optimal for DNA polymerases in vitro. We found that triplex formation causes specific termination of DNA polymerization in vitro. This effect is detected for different DNA polymerases and may have implications for the regulation of DNA replication in vivo.
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页码：11406 / 11410
页数：5
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