REGENERATION AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF CHRYSANTHEMUM

被引：12

作者：

LEDGER, SE ^{[1
]}

DEROLES, SC ^{[1
]}

GIVEN, NK ^{[1
]}

机构：

[1] MINIST AGR & FISHERIES,HORT RES CTR,LEVIN,NEW ZEALAND

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A method has been developed to regenerate shoots directly from leaf pieces of the autumn flowering chrysanthemum Dendranthema indicum (L.) Des Moul (genotype Korean). Transgenic plants of this genotype were generated using transformation mediated by the disarmed strain of Agrobacterium tumefaciens LBA4404, containing either pKIWI110 or pGA643. Both pKIWI110 and pGA643 contain the selectable marker gene neomycin phosphotransferase II (NPTII) and pKIWI110 also contains the reporter gene beta-D-glucuronidase. Leaf pieces inoculated with pKIWI110 produced zones of blue cells two days after inoculation. Shoots from leaf pieces inoculated with pGA643 were selected on kanamycin. PCR and Southern analysis of shoots that were able to root on kanamycin confirmed the presence of the NPTII gene in the plant genome.

引用

页码：195 / 199

页数：5

共 16 条

[1]

AN G, 1988, PLANT MOL BIOL MANUA, P1, DOI DOI 10.1007/978-94-009-0951-9

[2] RADIATION-INDUCED LOW-TEMPERATURE TOLERANT SOLID MUTANTS OF CHRYSANTHEMUM-MORIFOLIUM RAM [J].