EFFECTS OF ORGANIC PH BUFFERS ON A CELL-GROWTH AND AN ANTIBODY-PRODUCTION OF HUMAN-HUMAN HYBRIDOMA HB4C5 CELLS IN A SERUM-FREE CULTURE

被引：8

作者：

NAGIRA, K ^{[1
]}

HAYASHIDA, M ^{[1
]}

SHIGA, M ^{[1
]}

SASAMOTO, K ^{[1
]}

KINA, K ^{[1
]}

OSADA, K ^{[1
]}

SUGAHARA, T ^{[1
]}

MURAKAMI, H ^{[1
]}

机构：

[1] KYUSHU UNIV,GRAD SCH GENET RESOURCES TECHNOL,CELLULAR REGULAT TECHNOL LAB,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

CYTOTECHNOLOGY | 1995年 / 17卷 / 02期

关键词：

ANTIBODY PRODUCTION; CELL GROWTH; CHEMICAL STRUCTURE; HUMAN-HUMAN HYBRIDOMA; ORGANIC CONCEPTUAL DIAGRAM; ORGANIC PH BUFFER;

D O I：

10.1007/BF00749399

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human-human hybridoma cells secreting a human monoclonal antibody were cultured in a serum-free medium containing various organic pH buffers in order to clarify their effects on cell growth and antibody production. Organic pH buffers having either one sulfonic acid and several acyclic amine moieties, or several cyclic amine moieties containing two amino nitrogen did not inhibit cell growth; while other organic buffers sulfonic acid moiety plus several cyclic amine moieties containing one amino nitrogen slightly decreased cell growth, but enhanced antibody production. Using Fujita's organic conceptual diagram, a relationship between the organicity and inorganicity of a pH buffer to cell growth and antibody production was found. pH buffers with large inorganicity and small organicity values were favorable for cell growth, and buffers with small inorganicity and large organicity values were preferred to enhance antibody production. Although the pH buffering range affects cell growth, its effect on antibody production is not clear. In conclusion, 2-morpholinoethanesulfonic acid (MES), 3-morpholinopropanesulfonic acid (MOPS) and 1, 2-N, N'-bis[N '', N'''-di(2-sulfonoethyl)piperazinyl]ethane (Bis-PIPES) are shown to be the most optimal of the buffers tested, because they enhanced antibody production without decreasing the cell growth among the pH buffers tested here.

引用

页码：117 / 125

页数：9

共 22 条

[1] BUFFER COMBINATIONS FOR MAMMALIAN CELL CULTURE [J].