SEPARATION OF CHLOROPHYLL C(1) AND C(2) BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The separation of chlorophyll c(1) and c(2) was performed using an octadecyl-bonded polymer column with an aqueous methanolic mobile phase, e.g. a 90:10 (v/v) mixture of methanol and pH 3 buffer. The retention of both types of chlorophyll c increases with decreasing methanol content and also with decreasing pH (particularly lower than about pH 6) of the aqueous component of the mobile phase. Chlorophyll c(1) is eluted from the column in preference to chlorophyll c(2). UV-visible spectral data were obtained for the chlorophylls.