HIGH-RESOLUTION ORDERING OF YAC CONTIGS USING EXTENDED CHROMATIN AND CHROMOSOMES

被引：57
作者：
HAAF, T
WARD, DC
机构：
[1] Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, 333 Cedar Street
来源：
HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 04期
关键词：
D O I：
10.1093/hmg/3.4.629
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The general usefulness of fluorescence in situ hybridization (FISH) for the physical mapping of the human genome is greatly enhanced by improved DNA resolution. Several techniques have been described for decondensing or stretching interphase chromatin in a linear fashion, allowing long-range FISH mapping in finer detail. Highly extended linear chromatin can be hybridized over physical distances of at least several megabases, possibly over the whole length of a chromosome. By multi-color FISH, we have determined the order and overlaps of YACs from a 5q34 - 35 contig. Cosmids can be localized within larger YAC clones. Extended chromatin mapping can be applied as an adjunct ordering technique in genome studies.
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页码：629 / 633
页数：5
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