SIMPLIFIED, RAPID AND INEXPENSIVE EXTRACTION PROCEDURE FOR A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF DISOPYRAMIDE AND ITS MAIN METABOLITE MONO-N-DEALKYLATED DISOPYRAMIDE IN SERUM

被引：8

作者：

MAYER, F ^{[1
]}

KRAMER, BK ^{[1
]}

RESS, KM ^{[1
]}

KUHLKAMP, V ^{[1
]}

LIEBICH, HM ^{[1
]}

RISLER, T ^{[1
]}

SEIPEL, L ^{[1
]}

机构：

[1] UNIV TUBINGEN,MED KLIN,ABT 3,OTFRIED MULLER STR 10,W-7400 TUBINGEN 1,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 572卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80501-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simplified, rapid and inexpensive extraction procedure for the determination of the antiarrhythmic drug disopyramide and its main metabolite mono-N-desalkylated disopyramide in serum by high-performance liquid chromatography has been developed. The analysis uses ultraviolet detection at 254 nm, and a 5-mu-m reversed-phase column with a mobile phase of water-triethylamine-acetonitrile-PIC-B8 reagent. Serum extraction is performed with dichloromethane and 1 M sodium hydroxide. p-Chlorodisopyramide is used as internal standard. Recovery rates were 94.5% (S.D. 5.7%) for disopyramide, 96.8% (S.D. 2.2%) for mono-N-desalkylated disopyramide and 97.9% (S.D. 2.8%) for the internal standard.

引用

页码：339 / 345

页数：7

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