INVITRO PROTEIN TRANSLOCATION INTO INVERTED MEMBRANE-VESICLES PREPARED FROM VIBRIO-ALGINOLYTICUS IS STIMULATED BY THE ELECTROCHEMICAL POTENTIAL OF NA+ IN THE PRESENCE OF ESCHERICHIA-COLI SECA

被引:19
作者
TOKUDA, H [1 ]
KIM, YJ [1 ]
MIZUSHIMA, S [1 ]
机构
[1] UNIV TOKYO,INST APPL MICROBIOL,1-1-1 YAYOI,BUNKYO KU,TOKYO 113,JAPAN
关键词
Escherichia coli; Na[!sup]+[!/sup] pump; Protein secretion; SecA protein; Vibrio alginolyticus;
D O I
10.1016/0014-5793(90)80751-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na+ pump-possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na+ pump, suggesting that the electrochemical potential of Na+ but not that of H+, is important for protein translocation in Vibrio. © 1990.
引用
收藏
页码:10 / 12
页数:3
相关论文
共 14 条