ASTROCYTE DIFFERENTIATION IS ENHANCED IN CHICK-EMBRYOS TREATED WITH ETHANOL DURING EARLY NEUROEMBRYOGENESIS

In this study, we examined the effects of ethanol administered to chick embryos, on the maturation of astrocytes, using glutamine synthetase (GS) activity as an astrocyte marker. Ethanol (50 mM) was administered in ovo via the air sac, embryos were sacrificed at various days of embryonic development and GS activity was determined in cerebral hemispheres and cerebellum. We found that in both cerebral hemispheres and cerebellum, GS activity was higher in the ethanol-treated embryos, as compared to controls, during the embryonic periods, E6 to E10 in the cerebral hemispheres and E10 to E14 in the cerebellum. These periods are characterized by increased neuronal differentiation in these CNS areas. The increase in GS activity in the ethanol-treated embryos is speculated to reflect either a transient reactive gliosis and/or an enhancement in the differentiation of radial glia, immature glia, to more mature astrocytes.