IMMUNOHISTOCHEMICAL CHARACTERIZATION OF SMALL, LYMPHOID-LIKE CELL-POPULATIONS WITHIN GERMINOMAS - IMMUNOLOGICAL AND MOLECULAR APPROACHES TO DIAGNOSIS

被引：9

作者：

NITTA, T ^{[1
]}

HISHII, M ^{[1
]}

SATO, K ^{[1
]}

OKUMURA, K ^{[1
]}

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT IMMUNOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

CANCER LETTERS | 1995年 / 90卷 / 02期

关键词：

GERMINOMA; BRAIN; IMMUNOHISTOCHEMISTRY; T-CELL; ADHESION MOLECULES; T-CELL RECEPTOR;

D O I：

10.1016/0304-3835(95)99394-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Little is known to date about the biological and molecular characteristics of 'small lymphoid cells' within intracranial germinomas. Frozen sections from three germinoma specimens were evaluated immunohistochemically in order to identify phenotypic markers expressed on human lymphoid cells as well as intercellular adhesion molecules. In addition, T-cell receptor (TCR) variable alpha- and beta-chain mRNA expression was analyzed by polymerase chain reaction (PCR). The small cells stained faintly with anti-CDS in two specimens, but were negative for the T cell specific markers, CD2, CD3, CD7, and CD8, In addition, these cells were weakly positive for CD11b (Mac-1) and CD54 (ICAM-1), but were negative for lymphocyte-specific CD11a (LFA-I) and CD11c (p150,95). No TCR V alpha or V beta gene expression was detected by PCR within these germinoma specimens. The small cells of germinomas with the cytologic appearance of lymphocytes are not derived from T-cells or other lymphocytic lineages.

引用

页码：183 / 189

页数：7