Synthesis, characterization and biological activity studies on amide derivatives

In this study, with the intention of finding novel anti-biofilm and antiviral agents, a series of amide derivatives were synthesized, and their structures were elucidated by FT-IR and H-1-NMR and C-13-NMR and MS methods. Their purity was proven by TLC, HPLC and elemental analyses. Finally, the synthesized compounds were examined for their biofilm formation and swarming motility inhibitory activities in P. aeruginosa PA01. These compounds were found to reduce biofilm formation by 8.7-25.6% and swarming motility by 18.3-33.8% in P. aeruginosa PA01 at a concentration of 200 mu dM. Additionally, all the compounds were evaluated in terms of their antiviral activity against influenza A viruses. The plaque inhibition assays indicated that compound 6 (N-(4-{[5-(ethylamino)-1,3,4-thiadiazol-2-yl]methyl}phenyl)-4-fluorobenzamide) has a considerable inhibitory effect on influenza A virus plaque formation.