CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PSOPHOCARPIN-B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN-SEEDS

被引:11
作者
DATTAGUPTA, JK [1 ]
CHAKRABARTI, C [1 ]
PODDER, A [1 ]
机构
[1] INDIAN INST CHEM BIOL,DIV BIOCHEM ENGN,CALCUTTA 700032,W BENGAL,INDIA
关键词
D O I
10.1016/S0022-2836(05)80312-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Psophocarpin B1 is a 20,000 Mr protein of winged bean (Psophocarpus tetragonolobus) seeds having chymotrypsin inhibitory activity. Single crystals of this protein suitable for X-ray crystallographic studies have been obtained by the vapour diffusion method using ammonium sulphate. The crystals are hexagonal, space group P6422 or P6222, cell dimensions a=b=61 Å, c=210 Å. They are stable to irradiation with X-rays and diffract to at least 2·6 Å resolution. © 1990 Academic Press Limited.
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页码:229 / 231
页数:3
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