GENETIC SEGREGATION OF RANDOM AMPLIFIED POLYMORPHIC DNA IN DIPLOID CULTIVATED ALFALFA

被引：133
作者：
ECHT, CS
ERDAHL, LA
MCCOY, TJ
机构：
[1] Dept. Plant and Soil Science, Montana State University, Bozeman
来源：
GENOME | 1992年 / 35卷 / 01期
关键词：
RANDOM AMPLIFIED POLYMORPHIC DNA; POLYMERASE CHAIN REACTION AMPLIFICATION; GENOME MAPPING; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; MEDICAGO-SATIVA;
D O I：
10.1139/g92-014
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.
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页码：84 / 87
页数：4
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