GENETIC AND MOLECULAR ANALYSIS OF THE RPOD GENE FROM LACTOCOCCUS-LACTIS

被引：24
作者：
ARAYA, T
ISHIBASHI, N
SHIMAMURA, S
TANAKA, K
TAKAHASHI, H
机构：
[1] UNIV TOKYO, INST APPL MICROBIOL, 1-1 YAYOI 1 CHOME, BUNKYO KU, TOKYO 113, JAPAN
[2] MORINAGA MILK IND CO LTD, NUTR SCI LAB, KANAGAWA 228, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 01期
关键词：
D O I：
10.1271/bbb.57.88
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal sigma factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino add sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis sigma43 factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.
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页码：88 / 92
页数：5
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