ISOLATION OF CDNA-ENCODING A NOVEL SERPIN OF CRAYFISH HEMOCYTES

被引：28

作者：

LIANG, ZC ^{[1
]}

SODERHALL, K ^{[1
]}

机构：

[1] UNIV UPPSALA, DEPT PHYSIOL BOT, S-75236 UPPSALA, SWEDEN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1995年 / 112卷 / 02期

关键词：

SERPIN; PROTEINASE INHIBITOR; HEMOCYTE; FRESH-WATER CRAYFISH; CDNA;

D O I：

10.1016/0305-0491(95)00105-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a serpin-type proteinase inhibitor from a crayfish hemocyte cDNA library. The deduced amino acid sequence consists of 429 amino acids with a putative signal peptide of 21 amino acids, The mature protein has a calculated molecular mass of 45 029 daltons. Identities ranging up to 38% were observed between the crayfish serpin and other members of the serpin family, Phylogenetic analysis shows that the crayfish serpin has a closer relationship to insect serpins than to other animal serpins. Phe369-Ser370 were proposed to be the P1-P1' residues of the inhibitor reactive site, This protein was found to be expressed in hemocytes but not in the hepatopancreas of the crayfish Pacifastacus leniusculus.

引用

页码：385 / 391

页数：7

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