SEPARATION OF NEUTRAL MONOSACCHARIDES AND OLIGOSACCHARIDES DERIVATIZED WITH ETHYL PARA-AMINOBENZOATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON AN AMINE-BONDED VINYL ALCOHOL COPOLYMER COLUMN

被引：19

作者：

AKIYAMA, T ^{[1
]}

机构：

[1] NATL RES INST POLICE SCI,SEROL & BIOCHEM LAB,CHIYODA KU,TOKYO 102,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 588卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)85007-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A number of mono- and oligosaccharides derivatized with an ultraviolet-absorbing compound, ethyl p-aminobenzoate, were separated by high-performance liquid chromatography on an Asahipak NH2P-50 amine-bonded vinyl alcohol copolymer column. The derivatized mono- and oligosaccharides were sufficiently separated with isocratic elution and the separation efficiency for the derivatives was better than that for the underivatized counterparts. The column was remarkably stable relative to a conventional amine-bonded silica column and no decrease in the retention of the derivatives due to dissociation of the stationary phase was observed.

引用

页码：53 / 59

页数：7

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