IG/EBP (C/EBP-GAMMA) IS A TRANSDOMINANT NEGATIVE INHIBITOR OF C/EBP FAMILY TRANSCRIPTIONAL ACTIVATORS

被引：114
作者：
COOPER, C [1 ]
HENDERSON, A [1 ]
ARTANDI, S [1 ]
AVITAHL, N [1 ]
CALAME, K [1 ]
机构：
[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,NEW YORK,NY 10032
来源：
NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 21期
关键词：
D O I：
10.1093/nar/23.21.4371
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Analysis of cDNA and genomic clones shows that the murine lg/EBP (C/EBP gamma) gene encodes a small protein with a predicted molecular weight of 16.4 kDa which contains C/EBP family basic and leucine zipper domains but lacks the transcriptional activation domains present in C/EBP (C/EBP alpha)and NF-IL6 (C/EBP beta). In transfection assays Ig/EBP is neither an activator nor a repressor of transcription; however, Ig/EBP inhibits the transcriptional ability of NF-IL6 (C/EBP beta) and C/EBP (C/EBP alpha), acting as a transdominant negative regulator, Thus Ig/EBP resembles LIP, another negative regulator of the C/EBP family, in both structure and transcriptional activity, Of the three known C/EBP family inhibitors, Ig/EBP, LIP and CHOP-10, only Ig/EBP is ubiquitously expressed. Therefore, Ig/EBP may act as a general buffer for C/EBP activators in many cell types.
引用
收藏
页码：4371 / 4377
页数：7
相关论文
共 50 条
  • [41] Cooper C., Johnson D., Roman C., Avitahl N., Tucker P., Calame K., J. Immunol, 149, pp. 3225-3231, (1992)
  • [42] Tanaka T., Akira S., Yoshida K., Umemoto M., Yoneda Y., Shirafuji N., Fujiwara H., Suematsu S., Yoshida N., Kishimoto T., Cell, 80, pp. 353-361, (1995)
  • [43] Zhan Q., Lord K.A., Alamo I., Hollander M.C., Carrier F., Ron D., Kohn K.W., Hoffman B., Lieberman D.A., Fomace A.J., Mol. Cell. BioL, 14, pp. 2361-2371, (1994)
  • [44] Carlson S.G., Fawcett T.W., Bartlett J.D., Bemier M., Holbrook N.J., Mol. CelL BioL, 13, pp. 4736-4744, (1993)
  • [45] Vallejo M., Ron D., Miller C.P., Habener J.F., Proc. NaiL Acad. Sci. USA, 90, pp. 4679-4683, (1993)
  • [46] Fomace A.J., Nebert D.W., Hollander M.C., Leuthy J.D., Papathanasiou J., Faignoli J., Holbrook N.J., MoL CelL BioL, 9, pp. 4196-4203, (1989)
  • [47] Chiu R., Angel P., Karin M., Cell, 59, pp. 979-986, (1989)
  • [48] Nakabeppu Y., Nathans D., Cell, 64, pp. 751-759, (1991)
  • [49] Foulkes N., Sassone-Corsi P., Cell, 68, pp. 411-414, (1992)
  • [50] Foulkes N.S., Bonelli E., Sassone-Corsi P., Cell, 64, pp. 739-749, (1991)
12345