IG/EBP (C/EBP-GAMMA) IS A TRANSDOMINANT NEGATIVE INHIBITOR OF C/EBP FAMILY TRANSCRIPTIONAL ACTIVATORS

被引：114
作者：
COOPER, C [1 ]
HENDERSON, A [1 ]
ARTANDI, S [1 ]
AVITAHL, N [1 ]
CALAME, K [1 ]
机构：
[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,NEW YORK,NY 10032
来源：
NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 21期
关键词：
D O I：
10.1093/nar/23.21.4371
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Analysis of cDNA and genomic clones shows that the murine lg/EBP (C/EBP gamma) gene encodes a small protein with a predicted molecular weight of 16.4 kDa which contains C/EBP family basic and leucine zipper domains but lacks the transcriptional activation domains present in C/EBP (C/EBP alpha)and NF-IL6 (C/EBP beta). In transfection assays Ig/EBP is neither an activator nor a repressor of transcription; however, Ig/EBP inhibits the transcriptional ability of NF-IL6 (C/EBP beta) and C/EBP (C/EBP alpha), acting as a transdominant negative regulator, Thus Ig/EBP resembles LIP, another negative regulator of the C/EBP family, in both structure and transcriptional activity, Of the three known C/EBP family inhibitors, Ig/EBP, LIP and CHOP-10, only Ig/EBP is ubiquitously expressed. Therefore, Ig/EBP may act as a general buffer for C/EBP activators in many cell types.
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收藏
页码：4371 / 4377
页数：7
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