PREDICTION OF CHEMOTHERAPY RESPONSE IN HUMAN LEUKEMIA USING IN-VITRO CHEMOSENSITIVITY TEST

被引：16

作者：

HWANG, WS ^{[1
]}

CHEN, LM ^{[1
]}

HUANG, SH ^{[1
]}

WANG, CC ^{[1
]}

TSENG, MT ^{[1
]}

机构：

[1] UNIV LOUISVILLE,LOUISVILLE,KY 40292

来源：

LEUKEMIA RESEARCH | 1993年 / 17卷 / 08期

关键词：

CHEMOSENSITIVITY; ACUTE LEUKEMIA; MTT ASSAY; DYE EXCLUSION ASSAY;

D O I：

10.1016/0145-2126(93)90074-U

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We performed the dye exclusion assay (DEA) and the MTT dye reduction assay to determine the drug sensitivity of acute leukemia using short-term microplate cultures. The in vitro results were compared with the clinical response of 31 patients with acute leukemia treated by combination chemotherapy. The true-positive rates of the DEA and MTT assays were 86.7 and 91.3%, respectively; the true-negative rates were 33.3 and 77.8%, respectively; and the predictive accuracy was 62.5 and 87.5%, respectively. The DEA and MTT assays gave comparable results in drug sensitivity testing of leukemic blast cells. Our data suggest that MTT assay is the more suitable for assessing chemosensitivity in acute leukemia.

引用

页码：685 / 688

页数：4

共 20 条

[1] INVITRO DETERMINATION OF TUMOR CHEMOSENSITIVITY IN HEMATOLOGICAL MALIGNANCIES [J].