MEASUREMENT OF THE INDIVIDUAL PK(A) VALUES OF ACIDIC RESIDUES OF HEN AND TURKEY LYSOZYMES BY 2-DIMENSIONAL H-1-NMR

被引：139
|
作者：
BARTIK, K
REDFIELD, C
DOBSON, CM
机构：
[1] UNIV OXFORD,NEW CHEM LAB,OXFORD OX1 3QT,ENGLAND
[2] UNIV OXFORD,OXFORD CTR MOLEC SCI,OXFORD OX1 3QT,ENGLAND
[3] FREE UNIV BRUSSELS,B-1050 BRUSSELS,BELGIUM
来源：
BIOPHYSICAL JOURNAL | 1994年 / 66卷 / 04期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/S0006-3495(94)80900-2
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The pH dependence of the two-dimensional H-1 nuclear magnetic resonance spectra of hen and turkey egg-white lysozymes has been recorded over the pH range 1-7. By monitoring the chemical shifts of the resonances of the various protons of ionizable residues, individual pK, values for the acidic residues have been determined for both proteins. The pK(a) values are displaced, with the exception of those of the residues in the active site cleft, by an average of 1 unit to low pH compared to model compounds.
引用
收藏
页码：1180 / 1184
页数：5
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