SCANNING TUNNELING MICROSCOPY OF DNA - THE CHEMICAL MODIFICATION OF GOLD SURFACES FOR IMMOBILIZATION OF DNA

被引：42
作者：
BOTTOMLEY, LA
HASELTINE, JN
ALLISON, DP
WARMACK, RJ
THUNDAT, T
SACHLEBEN, RA
BROWN, GM
WOYCHIK, RP
JACOBSON, KB
FERRELL, TL
机构：
[1] OAK RIDGE NATL LAB,DIV CHEM,OAK RIDGE,TN 37830
[2] OAK RIDGE NATL LAB,DIV HLTH & SAFETY RES,OAK RIDGE,TN 37830
[3] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37830
来源：
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS | 1992年 / 10卷 / 04期
关键词：
D O I：
10.1116/1.577735
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
We have investigated the coulostatic immobilization of DNA to a chemically modified gold surface. The chemical modification involves the covalent attachment of chemically polarizable groups by reaction of thiols and disulfides with clean gold surfaces. We have determined that chemically modified surfaces possessing pendant cationic groups bind DNA. In this paper we report the reliable and reproducible scanning tunneling microscopy (STM) imaging of DNA using a gold surface chemically modified with N,N,-dimethyl-2-mercaptoethylamine and present the first STM images of an entire genetically functional DNA molecule, the circular plasmid pBS+.
引用
收藏
页码：591 / 595
页数：5
相关论文
共 25 条
[1]  
ALLISON DP, 1990, SCANNING MICROSCOPY, V4, P517
[2]  
ALLISON DP, IN PRESS ULTRAMICROS
[3]  
Bain C.D., 1989, ADV MATER, V4, P110
[4]   DIRECT OBSERVATION OF NATIVE DNA STRUCTURES WITH THE SCANNING TUNNELING MICROSCOPE [J].
BEEBE, TP ;
WILSON, TE ;
OGLETREE, DF ;
KATZ, JE ;
BALHORN, R ;
SALMERON, MB ;
SIEKHAUS, WJ .
SCIENCE, 1989, 243 (4889) :370-372
[5]  
Binnig G., 1984, TRENDS PHYSICS, P38
[6]  
BROWN GM, IN PRESS J ULTRAMICR
[7]   MOLECULAR-STRUCTURE OF DNA BY SCANNING TUNNELING MICROSCOPY [J].
CRICENTI, A ;
SELCI, S ;
FELICI, AC ;
GENEROSI, R ;
GORI, E ;
DJACZENKO, W ;
CHIAROTTI, G .
SCIENCE, 1989, 245 (4923) :1226-1227
[8]   IMAGING OF SINGLE-STRANDED-DNA WITH THE SCANNING TUNNELING MICROSCOPE [J].
CRICENTI, A ;
SELCI, S ;
CHIAROTTI, G ;
AMALDI, F .
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (02) :1285-1287
[9]   GOLD GROWN EPITAXIALLY ON MICA - CONDITIONS FOR LARGE AREA FLAT FACES [J].
DEROSE, JA ;
THUNDAT, T ;
NAGAHARA, LA ;
LINDSAY, SM .
SURFACE SCIENCE, 1991, 256 (1-2) :102-108
[10]   ATOMIC-SCALE IMAGING OF DNA USING SCANNING TUNNELING MICROSCOPY [J].
DRISCOLL, RJ ;
YOUNGQUIST, MG ;
BALDESCHWIELER, JD .
NATURE, 1990, 346 (6281) :294-296
123