CLONING AND FUNCTIONAL EXPRESSION OF HUMAN CDNA FOR THE ETB ENDOTHELIN RECEPTOR

被引：272
作者：
SAKAMOTO, A
YANAGISAWA, M
SAKURAI, T
TAKUWA, Y
YANAGISAWA, H
MASAKI, T
机构：
[1] UNIV TSUKUBA,INST CLIN MED,SAKURA,IBARAKI 305,JAPAN
[2] UNIV TOKYO,DEPT INTERNAL MED 2,TOKYO 113,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期
关键词：
D O I：
10.1016/0006-291X(91)90158-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo. © 1991.
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收藏
页码：656 / 663
页数：8
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