DNA CLEAVAGE CATALYZED BY THE RIBOZYME FROM TETRAHYMENA

被引：122

作者：

HERSCHLAG, D ^{[1
]}

CECH, TR ^{[1
]}

机构：

[1] UNIV COLORADO,HOWARD HUGHES MED INST,DEPT CHEM & BIOCHEM,BOULDER,CO 80309

来源：

NATURE | 1990年 / 344卷 / 6265期

关键词：

D O I：

10.1038/344405a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An RNA enzyme derived from the self-splicing intervening sequence of Tetrahymena thermophila catalyses sequence-specific cleavage of an oligodeoxyribonucleotide substrate. Compared with RNA, the DNA substrate is bound very weakly and is cleaved very slowly, revealing the importance of the RNA 2'-hydroxyl group in both the binding and chemical steps. The finding that catalysis by RNA can extend to DNA substrates indicates new possibilities for the transposition of intervening sequences and for the design of DNA cleavage agents with novel sequence specificities. © 1990 Nature Publishing Group.

引用

页码：405 / 409

页数：5

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