FLP RECOMBINASE IS AN ENZYME

被引：38
作者：
GATES, CA
COX, MM
机构：
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1988年 / 85卷 / 13期
关键词：
D O I：
10.1073/pnas.85.13.4628
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The FLP protein of the yeast 2-.mu.m plasmid catalyzes intermolecular site-specific recombination with a turnover number of .apprxeq. 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalylic rather than stoichiometric amounts. The reaction rate rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.
引用
收藏
页码：4628 / 4632
页数：5
相关论文
共 36 条
[1]  
ABREMSKI K, 1984, J BIOL CHEM, V259, P1509
[2]   STUDIES ON THE PROPERTIES OF P1 SITE-SPECIFIC RECOMBINATION - EVIDENCE FOR TOPOLOGICALLY UNLINKED PRODUCTS FOLLOWING RECOMBINATION [J].
ABREMSKI, K ;
HOESS, R ;
STERNBERG, N .
CELL, 1983, 32 (04) :1301-1311
[3]   INTERACTION OF THE FLP RECOMBINASE OF THE SACCHAROMYCES-CEREVISIAE 2-MU-M PLASMID WITH MUTATED TARGET SEQUENCES [J].
ANDREWS, BJ ;
MCLEOD, M ;
BROACH, J ;
SADOWSKI, PD .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (07) :2482-2489
[4]   THE FLP RECOMBINASE OF THE 2-MU CIRCLE DNA OF YEAST - INTERACTION WITH ITS TARGET SEQUENCES [J].
ANDREWS, BJ ;
PROTEAU, GA ;
BEATTY, LG ;
SADOWSKI, PD .
CELL, 1985, 40 (04) :795-803
[5]  
BROACH JR, 1981, MOL BIOL YEAST SACCH, V1, P445
[6]  
BRUCKNER RC, 1986, J BIOL CHEM, V261, P1798
[7]   THE FLP PROTEIN OF THE YEAST 2-MU-PLASMID - EXPRESSION OF A EUKARYOTIC GENETIC-RECOMBINATION SYSTEM IN ESCHERICHIA-COLI [J].
COX, MM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (14) :4223-4227
[8]   COPY NUMBER AMPLIFICATION OF THE 2-MU-M CIRCLE PLASMID OF SACCHAROMYCES-CEREVISIAE [J].
FUTCHER, AB .
JOURNAL OF THEORETICAL BIOLOGY, 1986, 119 (02) :197-204
[9]  
GATES CA, 1987, UCLA S MOL CELL BIOL, V68, P197
[10]   COMMUNICATION BETWEEN SEGMENTS OF DNA DURING SITE-SPECIFIC RECOMBINATION [J].
GELLERT, M ;
NASH, H .
NATURE, 1987, 325 (6103) :401-411
1234